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Enzymes and Proteins

EZ 0001 Albumin, Nuclease Free (Bovine serum)

Monomer MW: 66 kDa

Purity: ³ 90% Stability: stable for years at 2-8 ° C

• Tested for exonuclease, endonuclease, ribonuclease and protease

• Supplied as an aqueous solution at neutral pH in 50 % glycerol at 50mg/ml

EZ 0002 Asparaginase (E.coli)

Tetramer MW: 141 kDa

Purity: ³ 98% Stability: ³ 12 months at 2-8° C

• Chromatographically purified

• Dialyzed and lyophilized powder

• Activity: 150 units per mg protein. 1 unit releases 1 m mole of ammonia from L-asparagine per minute at 37 ° C in 50 mM Tris-Cl, pH 8.6

EZ 0003 Carbonic Anhydrase (Bovine erythrocytes)

Monomer MW: 30 kDa

Purity: ³ 90% Stability: ³ 6-12 months at 2-8° C

• Dialyzed and lyophilized powder

• Activity: 3000 units per mg dry weight. 1 unit is defined as the time required (in seconds) for a saturated carbon dioxide solution to lower the pH of 0.002 M Tris-Cl buffer from 8.3 to 6.3 at 0° C

EZ 0004 Cellulase (Trichoderma reesei)

Complex

Purity: ³ 98% Stability: ³ 12-24 months at 2-8° C

• Chromatographically purified complex containing exo- and endoglucanase activities.

• Dialyzed, lyophilized powder

• Tested for lipase, protease and deoxyribonuclease

• Activity: 45 units per mg dry weight. 1 unit releases 0.01 mg of glucose per hour from microcrystalline cellulose at 37 ° C in 50mM acetic acid buffer, pH 5.0

EZ 0005 Catalase (Bovine liver)

Homotetramer MW: 250 kDa

Purity: ³ 98% Stability: ³ 12 months at 5° C

• A crystalline aqueous suspension of approximately 6 mg/ml containing thymol as a preservative.

• Activity: 40,000 units per mg protein. 1 unit decomposes 1 m mole of hydrogen peroxide per minute at 25 ° C in 50 mM phosphate buffer, pH 7.0

EZ 0006 Cholesterol esterase (Porcine pancreas)

Hexamer MW: 400 kDa

Purity: ³ 95% Stability: ³ 12 months at -20° C

• Chromatographically purified

• A lyophilized powder purified to minimize protease contamination

• Activity: 300 units/mg dry weight. 1 unit hydrolyses 1 m mole of cholesterol ester per minute at 37 ° C in 0.1 M phosphate buffer, pH 7.0

EZ 0007 Collagen, type I (Bovine achilles tendon)

Tropocollagen unit MW: 300 kDa

Purity: ³ 98% Stability: ³ 12-24 months at 2-8° C

• Chromatographically purified

• Supplied as a lyophilized powder

• <50 caseinase units per milligram

EZ 0008 Galactosidase, Beta (E.coli)

Tetramer MW: 540 kDa

Purity: ³ 75% Stability: ³ 6 months at 2-8° C

• Partially purified dried powder

• 50 units/mg dry weight. 1 unit hydrolyses 1 m mole of o-nitrophenyl-b-D-galactopyranoside per minute at 25° C in 0.1M sodium phosphate buffer, pH 7.5 with 1mM magnesium chloride

EZ 0009 Glucose Oxidase (Aspergillus niger)

Dimer MW: 160 kDa

Purity: ³ 98% Stability: ³ 12 months at 2-8° C

• Chromatographically purified with levels of cellobiase, lactase, amylase and sucrase < 0.1% and maltase <0.2 %

• Dialyzed and lyophilized powder

• 110 units/mg. 1 unit oxidizes 1 m mole of o-dianisidine per minute at 25° C in 0.1 M phosphate buffer, pH 6.0

Glutathione S-transferases

EZ 0010 Rat liver glutathione S-transferases

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 30 units/mg

• One unit will conjugate 1 mmol of 1-chloro-2,4-dinitro benzene with reduced glutathione per minute at pH 6.5 at 25° C.

• Contains mainly Ya, Yb, and Yc subunits

EZ 0011 Rat liver glutathione S-transferase Ya

Homodimer MW: 25 kDa/subunit

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 20 units/mg

• One unit will conjugate 1 mmol of 1-chloro-2,4-dinitro benzene with reduced glutathione per minute at pH 6.5 at 25° C

EZ 0012 Rat liver glutathione S-transferase Yb

Homodimer MW: 26.5 kDa/subunit

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 14 units/mg

• One unit will conjugate 1 mmol of 1-chloro-2,4-dinitro benzene with reduced glutathione per minute at pH 6.5 at 25° C

EZ 0013 Rat liver glutathione S-transferase Yc

Homodimer MW: 28 kDa/subunit

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 8 units/mg

• One unit will conjugate 1 mmol of 1-chloro-2,4-dinitro benzene with reduced glutathione per minute at pH 6.5 at 25° C

EZ 0014 Rat liver glutathione S-transferase Ya-Yc

Heterodimer MW: 25(Ya) and 28(Yc) kDa/subunit

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 18 units/mg

• One unit will conjugate 1 mmol of 1-chloro-2,4-dinitro benzene with reduced glutathione per minute at pH 6.5 at 25° C

EZ 0015 Selenium Glutathione Peroxidase

MW: 23 kDa

Purity: ³ 95% Stability: ³ 6 months at -80° C

• Specific activity 300-500 units/mg

• One unit will catalyse the oxidation of 1mmol of reduced glutathione per minute at pH 7.0 at 25° C.

• GSH-Px from rat liver

EZ 0016 Human c-Jun (1-72)-GST

GST-fusion to human c-Jun (residues 1-72) MW: 36 kDa/subunit

Purity: ³ 92% Stability: ³ 1 year at -20° C

Glutathione-agarose Chromatography

Application: Protein kinase assay, western immunoblotting

Properties: Substrate for SAPK1/JNK

EZ 0017 Human GST-Bad (full length cDNA)

GST-fusion to human Bad (full length) MW: 28 kDa/subunit

Purity: ³ 92% Stability: ³ 1 year at -20° C

Glutathione-agarose Chromatography

Application: Protein kinase assay, western immunoblotting

Properties: Substrate for AKT

EZ 0018 Hyaluronidase (Bovine testes)

Tetramer MW: 55 kDa

Purity: ³ 98% Stability: ³ 6 12 months at 2-8° C

• Chromatographically purified

• Dialyzed and lyophilized powder

• 3000 units/mg. 1 unit corresponds to a USP/National Formulatory (NFXII) unit and is referenced to a standard NF hyaluronidase

EZ 0019 Lactate dehydrogenase (Bovine heart)

Tetramer MW: 137 kDa

Purity: ³ 98% Stability: ³ 6-12 months at 2-8° C

• Chromatographically purified

• Supplied as a suspension in 2.2 M ammonium sulfate

• 200 units/mg. 1 unit oxidises 1 m mole of NADH per minute at 25 ° C 0.15 M Tris-Cl buffer, pH 7.3

EZ 0020 5-Lipoxygenase (Potato Tubers)

Monomer MW: 85 kDa

Purity: ³ 98% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfate

• 15,000 units/mg. 1 unit = 1 nmole of oxygen consumed per minute at 25° C in 0.1 M phosphate buffer, pH 6.3, containing 200 mM linoleate

EZ 0021 5-Lipoxygenase (Potato Tubers) L₁

Monomer MW: 85 kDa

Purity: ³ 99% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfate

• 15,000 units/mg. 1 unit = 1 nmole of oxygen consumed per minute at 25° C in 0.1 M phosphate buffer, pH 6.3, containing 200 mM linoleate

EZ 0022 5-Lipoxygenase (Potato Tubers) L₂

Monomer MW: 85 kDa

Purity: ³ 99% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfate

• 15,000 units/mg. 1 unit = 1 nmole of oxygen consumed per minute at 25° C in 0.1 M phosphate buffer, pH 6.3, containing 200 mM linoleate

EZ 0023 5-Lipoxygenase (Potato Tubers) L₃

Monomer MW: 85 kDa

Purity: ³ 99% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfate

• 15,000 units/mg. 1 unit = 1 nmole of oxygen consumed per minute at 25° C in 0.1 M phosphate buffer, pH 6.3, containing 200 mM linoleate

EZ 0024 5-Lipoxygenase (Expressed in E. coli)

Monomer MW: 85 kDa

Purity: ³ 99% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M phosphate buffer, pH 6.3, containing 2 M ammonium sulfate

• 15,000 units/mg. 1 unit = 1 nmole of oxygen consumed per minute at 25° C in 0.1 M phosphate buffer, pH 6.3, containing 200 mM linoleate

EZ 0025 12-Lipoxygenase (Porcine Leukocytes)

Monomer MW: 72 kDa

Purity: ³ Semi-purified Stability: ³ 1 year at -80° C

• Supplied in 20 mM phosphate buffer, pH 7.5

• One unit of enzyme consumes one nanomole of oxygen per minute at 25° C in 0.1 M Tris-HCl buffer, pH 7.5, containing 5 mM EDTA, 0.03% Tween 20, and 100 mM arachidonate.

EZ 0026 15-Lipoxygenase (Soybean Type I)

Monomer MW: 94 kDa

Purity: ³ 98% Stability: ³ 6 months at -80° C

• Supplied in 0.1 M Tris-HCl, pH 7.0, with 10% glycerol

• Specific activity: 150,000-300,000 units/mg

• 1 unit will cause an increase in A₂₃₄ of 0.001/min at pH 9.0 at 25° C when linoleate acid is the substrate in 3.0 ml volume.

• Isolated from ram seminal vesicles.

EZ 0027 Lysozyme (Egg white)

Monomer MW: 14 kDa

Purity: ³ 98% Stability: ³ 3 years at 2-8° C

• Two times crystallized preparation

• A lyophilized preparation containing sodium chloride and acetate

• 5000 units/mg. 1 unit causes a decrease in turbidity at 450nm of 0.001 per minute at 25° C in 0.1 M phosphate buffer, pH 7.0

EZ 0028 Malate dehydrogenase (Porcine heart mitochondria)

Dimer MW: 70 kDa

Purity: ³ 98% Stability: ³ 12 months at 2-8° C

• Chromatographically purified

• Supplied as a suspension in 3.2 M ammonium sulfate

• 1000 units/mg. 1 unit oxidizes 1 m mole of NADH per minute at 25 ° C in 0.1 M phosphate buffer, pH 7.4

EZ 0029 Neuraminidase (Clostiridium perfringens)

Purity: ³ 70% Stability: ³ 2 years at 2-8° C

• Lyophilized powder

• 0.5 units/mg. 1 unit releases 1 m mole of sialic acid per minute at 37 ° C in 0.1 M acetate buffer, pH 5.0.

EZ 0030 Nuclease, S1 (Aspergillus oryzae)

Monomer MW: 34 kDa

Purity: ³ 95% Stability: ³ 6 months at -20° C

• Chromatographically purified

• Supplied as a solution in 30 mM sodium acetate, pH 4.6, containing 50 mM sodium chloride, 1mM zinc chloride and 50 % glycerol

• 100,000 units/ml. 1 unit renders acid soluble 1 m g of denatured DNA per minute at 37 ° C in 30 mM sodium acetate buffer, pH 4.6

EZ 0031 Papain (Papaya Latex)

Monomer MW: 23 kDa

Purity: ³ 98% Stability: ³ 6-12 months at 2-8° C

• supplied as a lyophilized powder prepared from a crystalline suspension

• To ensure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067 mM b-mercaptoethanol and 5.5 mM cysteine HCl for 30 minutes.

• 15 units/mg. 1 unit hydrolyzes 1 m mole of benzoyl-L-arginyl ethyl ester (BAEE) per minute at 25° C at pH 6.3

EZ 0032 Peroxidase, Horseradish

(Horse radish roots)

Monomer MW: 40 kDa

Purity: ³ 98% Stability: ³ 9-12 months at 2-8° C

• Chromatographically purified

• Lyophilized powder

• Single isozyme with RZ>2.9

• 500 units/mg. 1 unit decomposes 1 m mole of hydrogen peroxide per minute at 25° C in 0.1 M phosphate buffer, pH 7.0, containing aminoantipyrine and phenol

EZ 0033 Phosphatase, Acid (Wheat germ)

Monomer MW: 55 kDa

Purity: ³ 70% Stability: ³ 9-12 months at 2-8° C

• Partially purified preparation

• 15 units/mg. 1 unit hydrolyzes 1 m mole of o-carboxy-(or p-nitro) phenyl-phosphate per minute at 25 ° C in 50 mM sodium acetate buffer, pH 5.0

EZ 0034 Phosphatase, Alkaline (Calf intestine)

MW: 140 kDa

Purity: ³ 90% Stability: ³ 6 months at 2-8° C

• Chromatographically purified

• Supplied as a solution in 3 M sodium chloride containing 1 mM magnesium chloride, 0.1 mM zinc chloride and 0.3 M TEA, pH 7.6, at a concentration of 10mg/ml

• 3000 units/mg. 1 unit hydrolyzes 1 m mole of p-nitrophenol phosphate per minute at 25 ° C, pH 9.8

EZ 0035 Proteinase K (Tritirachium albumlimber)

Monomer MW: 29 kDa

Purity: ³ 90% Stability: ³ 12 months at 2-8° C

• Lyophilized powder

• Purified to remove DNase and RNase

• 20 units/mg. 1 unit releases 1 m mole of Folin positive amino acids per minute, measured as tyrosine, at 37° C, pH 7.5, using urea denatured hemoglobin as the substrate

EZ 0036 Prostaglandin H Synthase 1 (Ovine)

[COX-1]

Homodimer MW: 70 kDa/subunit

Purity: ³ 95% Stability: ³ 2 years at -80° C

• Supplied in 80 mM Tris-HCl, pH 8.0, with 0.1% Tween 20 and 300 µM DDC

• Specific activity 40,000 units/mg

• One unit of enzyme consumes 1nmole of oxygen per minute at 37° C in 0.1 M Tris-HCl, pH 8.0, containing 100 mM arachidonic acid, 5 mM EDTA and 2 mM phenol.

EZ 0037 Prostaglandin H Synthase 2 (Ovine)

[COX-2]

Homodimer MW: 72 kDa/subunit

Purity: ³ 70% Stability: ³ 1 year at -80° C

• Supplied in 80 mM Tris-HCl, pH 8.0, with 0.1% Tween 20 and 300 µM DDC

• Specific activity 1500 units/mg

• One unit of enzyme consumes 1 nmole of oxygen per minute at 37° C in 0.1M Tris-HCl, pH 8.0, containing 100 mM arachidonic acid, 5 mM EDTA and 2 mM phenol.

• Isolated from sheep placenta.

EZ 0038 Trypsin (Bovine pancreas)

Monomer MW: 24 kDa

Purity: ³ 95% Stability: ³ 2-3 years at -20° C

• Two times crystallized

• Dialyzed against 1mM HCl and lyophilized

• 180 units/mg. 1 unit hydrolyzes 1 m mole of p-toulene-sulphonyl-L-arginine methyl ester per minute at 25° C in 50 mM Tris-Cl, pH 8.1, containing 10 mM calcium chloride

EZ 0039 Urease (Jack beans)

Hexamer MW: 480 kDa

Purity: ³ 70% Stability: ³ 12 months at -20° C

• Fractionated from crude jack bean meal extract

• Tested for ammonia

• A soluble, lyophilized preparation

• 45 units/mg. 1 unit releases 1 m mole of ammonia per minute at 25 ° C in a glutamate dehydrogenase coupled system.

Molecular Weight Markers-for SDS-Electrophoresis of Proteins

MW 0040 Mark 12 Wide range Standard

• The Mark 12 standard is an unstained protein standard that allows close approximation of molecular weight range. It is supplied ready-to-use for either Tris-Glycine or Tricine gels

• Coomassie, silver or other protein staining methods are required to visualize this standard

• Contains 12 polypeptides which resolve into sharp, tight bands in the range of 2.5 kDa to 200 kDa (Myosin, b-galactosidase, phosphorylase-b, BSA, glutamate dehydrogenase, lactate dehydrogenase, carbonic anhydrase, trypsin inhibitor, lysozyme, aprotinin, insulin B chain and insulin A chain)

• Ideal for molecular weight estimation

• No mixing required

• 1 ml per vial; sufficient for 200 applications of 5 m l each.

Molecular Biologicals

Penn Bio 10x Assay Buffers for restriction enzyme reactions

Penn Bio provides 10 X assay buffer with each restriction endonuclease o ensure optimal activity. Some restriction enzymes require BSA at a final concentration of 100 m g/ml. BSA is supplied as 10 mg/ml (100x) stock when required and should be added to the reaction mixture.

Note: for Not I buffer contains 0.01 % Triton X-100. All buffers are supplied in 1 ml vials. For the price listings, contact the manufacturer.

BF 0046 Diluent Buffers

Diluent buffers are for diluting the restriction enzymes when needed. Buffer composition and the list of restriction

enzymes with its appropriate dilution buffer are given below:

Buffer 1 (Apa I, Hae III, Kpn I, Nhe I, Ssp I, Bcl I, Hha I, Mbo I, Nru I, Spe I, Cla I, Hinc II, Mbo II, Pvu II, Xho I, Dra I, Hinf I, Mlu I, Sal I, Xma I, Eco RV, Hpa I, Msp I, Sau 3A, Hae II, Hpa II, Nco I, Sma I): 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

Buffer 2 (Alu I, Bgl I, Bgl II, Hind III Pvu I, Sac I, Taq I): 10 mM Tris-Cl (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

Buffer 3 (Bam HI, Eco RI, Not I, Pst I, Sfi I, Xba I): 10 mM Tris-Cl (pH 7.4), 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.15% Triton X 100 and 50 % glycerol

For the price listings contact the manufacturer.

Restriction Endonucleases

(Price list available from manufacturer)

RE 0047 Alu I (Arthrobacter luteus)

• Assay condition: Buffer L, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0048 ApaL I (Acetobacter pasteurianus)

• Assay condition: Buffer E + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0049 Bam HI (Bacillus amyloliquifaciens)

• Assay condition: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.05% Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0050 Bcl I (Bacillus caldolyticus)

• Assay condition: Buffer B, 50 ° C

• Test Substrate: unmethylated l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 80 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0051 Bgl I (Bacillus globigii)

• Assay condition: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0052 Bgl II (Bacillus globigii)

• Assay condition: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0053 C la I (Caryophanon latum)

• Assay condition: Buffer E+BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0054 Dra I (Deinococcus radiophilus)

• Assay condition: Buffer E+BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0055 Eco RI (Escherichia coli)

• Assay condition: Buffer E+BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 5 mM KPO₄ (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.2 % Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering information:

RE 0056 Eco RV (Escherichia coli)

• Assay Conditions: Buffer A + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 5 mM KPO₄ (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.2 % Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0057 Hae II (Haemophilus aegypticus)

• Assay Conditions: Buffer E + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0058 Hae III (Haemophilus aegypticus)

• Assay Conditions: Buffer E + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0059 Hha I (Haemophilus haemolyticus)

• Assay Conditions: Buffer E + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0060 Hinc II (Haemophilus influenzae Rc)

• Assay Conditions: Buffer B + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0061 Hind III (Haemophilus Influenzae Rd)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0062 Hinf I (Haemophilus influenzae)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0063 Hpa I (Haemophilus parainfluenzae)

• Assay Conditions: Buffer E, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0064 Hpa II (Haemophilus parainfluenzae)

• Assay Conditions: Buffer L, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0065 Kpn I (Klebsiella pneumoniae)

• Assay Conditions: Buffer L + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0066 Mbo I (Moraxella bovis)

• Assay Conditions: Buffer B, 37 ° C

• Test Substrate: unmethylated l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0067 Mbo II (Moraxella bovis)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0068 Mlu I (Micrococcus luteus)

• Assay Conditions: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0069 Msp I (Moraxella species)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0070 Nco I (Nocardia corallina)

• Assay Conditions: Buffer E, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0071 Not I (Nocadia otidiscaviarum)

• Assay Conditions: Buffer A with 0.01 % Triton X-100 + BSA,

• 37 ° C

• Test Substrate: pDM 148

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.15 % Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0072 Nhe I (Niesseria mucosa heidelbergensis)

• Assay Conditions: Buffer L + BSA, 37 ° C

• Test Substrate: Eco RI / l DNA fragments

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0073 Nru I (Nocardia rubra)

• Assay Conditions: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0074 Pst I (Providentia stuartii)

• Assay Conditions: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.15% Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0075 Pvu I (Proteus vulgaris)

• Assay Conditions: Buffer B, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0076 Pvu II (Proteus vulagris)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0077 Sac I (Streptomyces achromogenes)

• Assay Conditions: Buffer L, 37 ° C

• Test Substrate: Eco RI / l DNA fragments

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0078 Sal I (Streptomyces albus G)

• Assay Conditions: Buffer A+BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0079 Sau 3AI (Staphylococcus aureus 3AI)

• Assay Conditions: Buffer E+BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0080 Sfi I (Streptomyces fimbriatus)

• Assay Conditions: Buffer E + BSA, 50 ° C

• Test Substrate: l DNA

• Storage Buffer: 5 mM KPO4 (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.15% Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0081 Sma I (Serratia marcescens)

• Assay Conditions: Buffer E, 25 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0082 Spe I (Sphaerotilus species)

• Assay Conditions: Buffer C + BSA, 37 ° C

• Test Substrate: Ad-2 DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0083 Ssp I (Sphaerotilus species)

• Assay Conditions: Buffer C, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0084 Taq I (thermus aquaticus YTI)

• Assay Conditions: Buffer B + BSA, 65 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 300 mM KCl, 0.1 mM EDTA, 1 mM DTT, 500 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0085 Xba I (Xanthomonas badrii)

• Assay Conditions: Buffer C + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA, 0.1 % Triton X-100 and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0086 Xho I (Xanthomonas holicida)

• Assay Conditions: Buffer E + BSA, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 10 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

RE 0087 Xma I (xanthomonasmalvacearum)

• Assay Conditions: Buffer L, 37 ° C

• Test Substrate: l DNA

• Storage Buffer: 20 mM Tris-Cl (pH 7.4), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 m g/ml nuclease free BSA and 50 % glycerol

• Store at –20 ° C

• Frequency of cutting:

• Ordering Information

DNA Modifying Enzymes

EZ 0088 Deoxyribonuclease I (Bovine pancreas)

Monomer MW: 31 kDa

Purity: ³ 98% Stability: ³ 2-3 years at 2-8° C

• Molecular biology grade

• Chromatographically purified to remove RNase and protease

• Lyophilized powder

• 10,000 units/mg. 1 unit causes an increase in absorbance at 260 nm of 0.001 per min per ml at 25 ° C when acting upon highly polymerised DNA in 0.1 M sodium acetate buffer, pH 5.0

EZ 0089 DNA Ligase, T4 (E.coli NM989)

Monomer MW: 68 kDa

Purity: ³ 90% Stability: ³ 6-12 months at -20° C

• Supplied as a solution in 10mM Tris-Cl, pH 7.6 with 1mM DTT, 5 mM KCl and 20 % glycerol

• 2000 units/mg. 1 unit catalyses the excahnge of 1nmole of ³²P from pyrophosphate into an activated carbon adsorbable compound

EZ 0090 DNA Polymerase I, Klenow Fragment (E.coli CM5199)

Monomer MW: 62 kDa

Purity: ³ 98% Stability: ³ 12 months at -20° C

• Chromatographically purified to remove DNase, RNase and endonuclease

• Supplied as a solution in 50mM potassium phosphate buffer, pH 7.4, containing 1mM DTT and 20 % glycerol

• Dilute the preparation in the above mentioned buffer with 0.1% BSA

• 2000 units/mg. 1 unit incorporates 10 nmoles of total deoxynucleotides into acid insoluble products with activated DNA as template primer in 30 minutes at 37 ° C

DNA Polymerase, Taq (Thermus aquaticus)

EZ 0091

Monomer MW: 95 kDa

Purity: ³ 98% Stability: ³ 6-12 months at -80° C

• Supplied in 50 mM potassium phosphate buffer, pH 7.4, containing 1mM DTT and 15 % glycerol

• 5,000 units/mg. Assay protocol same as that for DNA polymerase I

EZ 0092 DNA topoisomerase (Wheat germ)

Purity: ³ 98% Stability: ³ 6 months at -20° C

• Assay buffer: 50 mM Tris-Cl (pH 7.9), 1 mM EDTA, 50 mM NaCl, 1 mM DTT and 100 mg/ml nuclease free BSA

• Storage buffer: 50 mM Tris-Cl (pH 7.9), 1 mM EDTA, 500 mM NaCl, 10 mM b-mercaptoethanol, 200 mg/ml nuclease free BSA, 0.1 % Triton X-100 and 50 % glycerol.

• One unit is the amount of enzyme which will fully relax 1 mg of supercoiled pBR 322 plasmid DNA in 30 min at 37 ° C in a volume of 20 ml.

EZ 0093 T7 DNA polymerase (Wheat germ)

Purity: ³ 98% Stability: ³ 6 months at -20° C

• Assay buffer: 20 mM Tris-Cl (pH 7.5), 10 MgCl₂, 1 mM DTT and 50 mg/ml nuclease free BSA

• Storage buffer: 20 mM KPO4 (pH 7.4), 0.1 mM EDTA, 1 mM DTT and 50 % glycerol.

• One unit is defined as the amount of enzyme required to convert 10 n moles of total deoxyribonucleotides into an acid insoluble form in 30 minutes at 37 ° C

EZ 0094 Polynucleotide Kinase, T4 (E.coli infected with T4phage)

Purity: ³ 98% Stability: ³ 6-12 months at -20° C

• A highly purified preparation

• Supplied as a solution in 50 mM Tris-Cl buffer, pH 7.5, containing 1 mM DTT and 20 % glycerol

• 10,000 units/mg. 1 unit incorporates 1 nmole of g-32P from [g³²P] ATP into micrococcal nuclease treated calf thymus DNA in 30 minutes at 37 ° C

EZ 0095 Ribonuclease I (Bovine pancreas)

Monomer MW: 15 kDa

Purity: ³ 98% Stability: ³ 2 years at 2-8° C

• Molecular biology grade. Free of proteinase and DNase activities

• A solution containing approximately 5 mg/ml in 50 % glycerol

• Prepared specifically for purifying DNA plasmids

• 2000 units/mg. 1 unit causes an increase in absorbance of 1.0 at 260 nm at 37 ° C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oligonucleotides.

EZ 0096 RNA Polymerase, T7 (E.coli containing the bacteriophage T7 gene)

Monomer MW: 98 kDa

Purity: ³ 98% Stability: ³ 6 months at -20° C

• A highly purified preparation in 50 mM potassium phosphate buffer, pH 7.4, containing 1 mM DTT and 20 % glycerol

• 8000 units/mg. 1 unit of enzyme activity catalyzes the incorporation of 1 nanomole of labeled nucleoside monophosphate into acid insoluble products in 60 minutes at 25° C

EZ 0097 AMV Reverse Transcriptase (Avian myeloblastosis virus)

Purity: ³ 98% Stored at: -20° C

• Assay buffer: 50 mM Tris-Cl (pH 8.3), 8 mM MgCl₂, 1 mM DTT and 50 mM NaCl

• Storage buffer: 200 mM KPO₄ (pH 7.2), 2 mM DTT, 0.2 % Triton X-100 and 50 % glycerol.

• One unit is defined as the amount of enzyme which incorporates 1nmole of ³H TTP into an acid insoluble product in 10 minutes at 37 ° C using poly A-oligo dT as template-primer.

DNA Markers

MW 0098 DNA Molecular Weight Marker (100 bp ladder)

• The 100 bp ladder contains 10 bands of double stranded DNA fragment DNA fragment which ranges from 100 to 1000 base pairs. These are suitable for sizing of DNA fragments generated by PCR or restriction digests separated on agarose gels. On agarose gel electrophoresis the mixture separates into ten individual bands having the following number of base pairs: 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0099 Lambda DNA BstE II Fragments (E.coli lambda DNA)

• DNA fragments prepared by the digestion of lambda DNA with the restriction endonuclease Bst II and Exo-free Klenow DNA polymerase to stabilize the cohesive ends. On agarose gel electrophoresis the mixture separates into 14 individual bands having the following base pairs: 8454, 7242, 6369, 5686, 4822, 3775, 2323, 1929, 1371, 1264, 702, 224 and 117. A solution in 10 mM Tris-Cl, pH 8.0, with 1 mM EDTA.

• Store at -20° C

MW 0100 Lambda DNA, EcoR I fragments

• DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 21226, 7421, 5804, 4878 and 3530.

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 1mM EDTA

• Store at -20° C

MW 0101 Lambda DNA, Hind III fragments

• DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III. On agarose gel electrophoresis the mixture separates into five individual bands having the following number of base pairs: 23130, 9416, 6557, 4361, 2027 and 564.

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 1mM EDTA

• Store at -20° C

MW 0102 Lambda DNA/EcoRI + Hind III double digest

• DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonucleases Eco RI and Hind III. On agarose gel electrophoresis the mixture separates into thirteen individual bands having the following number of base pairs: 21226, 5148, 4973, 4277, 3530, 2027, 1904, 1584, 1330, 983, 831, 564, 125.

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0103 Lambda DNA Mlu I digest

• DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Mlu I. On agarose gel electrophoresis the mixture separates into eight individual bands having the following number of base pairs: 26282, 9824, 5090, 2419, 2205, 1268, 956 and 458.

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0104 pBR 322 DNA / Hinf I digest

• DNA fragments prepared by the digestion of purified pBR322 plasmid DNA with the restriction endonuclease Hinf I. On agarose gel electrophoresis the mixture separates into ten individual bands having the following number of base pairs: 1631, 517, 508, 396, 344, 298, 221, 220, 154 and 75

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0105 pBR 322 DNA / Hae III digest

• DNA fragments prepared by the digestion of purified pBR322 plasmid DNA with the restriction endonuclease Hae III. On agarose gel electrophoresis the mixture separates into twenty two individual bands having the following number of base pairs: 587, 540, 504, 458, 434, 267, 234, 213, 192, 184, 124, 123, 104, 89, 80, 64, 57, 51, 21, 18, 11 and 7.

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0106 pBR 322 DNA / Msp I digest

• DNA fragments prepared by the digestion of purified pBR322 plasmid DNA with the restriction endonuclease Msp I. On agarose gel electrophoresis the mixture separates into twenty six individual bands having the following number of base pairs: 622, 527, 404, 309, 242, 238, 217, 201, 190, 180, 160, 160, 147,147, 123, 110, 90, 76, 67, 34, 34, 26, 26, 15, 15, 9, 9

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0107 Phi X 174 DNA / Hae III digest

• DNA fragments prepared by the digestion of purified Phi X 174 DNA with the restriction endonuclease Hae III. On agarose gel electrophoresis the mixture separates into twenty six individual bands having the following number of base pairs: 622, 527, 404, 309, 242, 238, 217, 201, 190, 180, 160, 160, 147,147, 123, 110, 90, 76, 67, 34, 34, 26, 26, 15, 15, 9, 9

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0108 Phi X 174 DNA / Hinf I digest

• DNA fragments prepared by the digestion of purified Phi X 174 DNA with the restriction endonuclease Hinf I. On agarose gel electrophoresis the mixture separates into twenty individual bands having the following number of base pairs: 726, 713, 553, 500, 427, 417, 413, 311, 249, 200, 151, 140, 118, 100, 82, 66, 48, 42, 40, 24

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0109 pUC 18 / Sau3AI- pUC 18 /Taq I digest

• DNA fragments prepared by the digestion of purified pUC 18 DNA with the restriction endonuclease Sau3AI & Taq I separately. On agarose gel electrophoresis the mixture separates into nineteen individual bands having the following number of base pairs: 1444, 955, 736, 585, 476, 341, 258, 141, 105, 78, 75, 46, 36, 30, 18, 17, 12, 11, 8

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

MW 0110 Lamda/Hind III, pUC 18/Sau3AI-pUC18/Taq I digest

• DNA fragments prepared by the digestion of purified lambda with Hind III and pUC 18 DNA digested separately with Sau3AI and Taq I. On agarose gel electrophoresis the mixture separates into twenty seven individual bands having the following number of base pairs: 23130, 9416, 6557, 4361, 2322, 2027, 1444, 955, 736, 585, 564, 476, 341, 258, 141, 125, 105, 78, 75, 46, 36, 30, 18, 17, 12, 11, 8

• Supplied in 10 mM Tris-Cl, pH 8.0, containing 10 mM EDTA

• Store at -20° C

Dephosphorylated Vectors "Ready to clone"

• Penn Bio has selected two cloning vehicles among the most widely used. These vectors are offered in a linearized and dephosphorylated form.

• Dephosphorylation is checked by ligation and subsequent transformation (background); Integrity of the cloning site after linearization checked by kination and subsequent ligation recutting assay.

• Store at -20° C

CALL (518)-605-8808 or FAX: (206)-337-1074 FOR ORDERING